Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo.
Luo W, Mizuno H, Iwata R, Nakazawa S, Yasuda K, Itohara S, Iwasato T
Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 85006 | pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova) | For Cre/loxP-based Supernova-GFP, pK038 should be used with pK031. The GFP fragment of pK038 can be replaced with another gene by using SalI and EcoRV sites. |
| 85007 | pK036.TRE-Flpe-WPRE (Supernova) | For Flpe/FRT-based Supernova, pK036 should be used with pK068 etc. |
| 85008 | pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova) | For Flpe/FRT-based Supernova-GFP, pK068 should be used with pK036. The GFP fragment of pK068 can be replaced with another gene by SalI and EcoRV. |
| 85038 | pK037.CAG-FRT-stop-FRT-RFP-ires-tTA-WPRE (Supernova) | For Flpe/FRT-based Supernova-RFP, pK037 should be used with pK036. |
| 85039 | pK168.AAV-EF1α-DIO-tTA-P2A-RFP-WPRE (Supernova) | For AAV-TRE-Cre based Supernova-RFP, pK168 should be used with pK170. |
| 85040 | pK170.AAV-TRE-Cre-WPRE (Supernova) | For AAV-TRE-Cre based Supernova, pK170 should be used with pK168 etc. |
| 85041 | pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova) | This plasmid should be used for Cre/loxP-based Supernova-CRISPR/Cas9. |
| 85179 | pK098.CAG-loxP-stop-loxP-nlstagRFP-ires-tTA-WPRE (Supernova) | For nucleus-specific RFP labeling in single cells, pK098 should be used with pK031. |
| 89587 | pK263.CAG-loxP-stop-loxP-NLSturboRFP-ires-tTA-WPRE | For nucleus-specific turboRFP labeling in single cells, pK263 should be used with pK031. |