NAIP proteins are required for cytosolic detection of specific bacterial ligands in vivo.
Rauch I, Tenthorey JL, Nichols RD, Al Moussawi K, Kang JJ, Kang C, Kazmierczak BI, Vance RE
J Exp Med. 2016 May 2;213(5):657-65. doi: 10.1084/jem.20151809. Epub 2016 Apr 4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 84863 | pET22b - PA | bacterial expression of Bacillus anthracis protective antigen |
| 84866 | pFastBacDual with LFn + Fla | Baculovirus Expression of LFn -Fla fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein |
| 84867 | pFastBacDual with LFn + FlaAAA | Baculovirus Expression of LFn-FlaAAA fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine. |
| 84868 | pFastBacDual with LFn + Needle | Baculovirus Expression of LFn-Needle fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein |
| 84869 | pFastBacDual with LFn + PrgJ | Baculovirus Expression of LFn-PrgJ fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein |
| 84870 | pFastBacDual with PA+Histag | Baculovirus Expression of Bacillus anthracis protective antigen |
| 84871 | pET15b LFn-Fla | Bacterial expression of LFn-Fla fusion |
| 84872 | pET15b LFn-Fla 3A | Bacterial expression of LFn-Fla 3A fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine. |