New vectors for simple and streamlined CRISPR-Cas9 genome editing in Saccharomyces cerevisiae.

Laughery MF, Hunter T, Brown A, Hoopes J, Ostbye T, Shumaker T, Wyrick JJ
Yeast. 2015 Dec;32(12):711-20. doi: 10.1002/yea.3098. Epub 2015 Sep 21. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
67641	pJH001	High copy Cas9 expression vector with LEU2 marker for yeast transformation
67640	pT040	URA3 vector for cloning guide sequences using BclI-SwaI sites into guide RNA expression cassette
67639	pML107	Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation
67638	pML104	Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation