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An extra dimension in protein tagging by quantifying universal proteotypic peptides using targeted proteomics.

Vandemoortele G, Staes A, Gonnelli G, Samyn N, De Sutter D, Vandermarliere E, Timmerman E, Gevaert K, Martens L, Eyckerman S
Sci Rep. 2016 Jun 6;6:27220. doi: 10.1038/srep27220. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
84883pAav-MCS-PQS1-3xFLAGrAAV-based template for genome engineering of protein C-termini containing PQS1 and 3xFLAG tags and a selection cassette
84884pAav-TP53-PQS1-3xFLAGrAAV-based template for genome engineering of the p53 C-terminus containing PQS1 and 3xFLAG tags and a selection cassette
84885pAav-IQGAP1-PQS1-3xFLAGrAAV-based template for genome engineering of the IQGAP1 C-terminus containing PQS1 and 3xFLAG tags and a selection cassette
84886pAav-MDM2-PQS2-3xHArAAV-based template for genome engineering of the MDM2 protein C-terminus containing PQS2 and 3xHA tags and a selection cassette
84917pAav-MCS-PQS2-3xHArAAV-based template for genome engineering of protein C-termini containing PQS2 and 3xHA tags and a selection cassette

Antibodies from Article