An extra dimension in protein tagging by quantifying universal proteotypic peptides using targeted proteomics.
Vandemoortele G, Staes A, Gonnelli G, Samyn N, De Sutter D, Vandermarliere E, Timmerman E, Gevaert K, Martens L, Eyckerman S
Sci Rep. 2016 Jun 6;6:27220. doi: 10.1038/srep27220.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
84883 | pAav-MCS-PQS1-3xFLAG | rAAV-based template for genome engineering of protein C-termini containing PQS1 and 3xFLAG tags and a selection cassette |
84884 | pAav-TP53-PQS1-3xFLAG | rAAV-based template for genome engineering of the p53 C-terminus containing PQS1 and 3xFLAG tags and a selection cassette |
84885 | pAav-IQGAP1-PQS1-3xFLAG | rAAV-based template for genome engineering of the IQGAP1 C-terminus containing PQS1 and 3xFLAG tags and a selection cassette |
84886 | pAav-MDM2-PQS2-3xHA | rAAV-based template for genome engineering of the MDM2 protein C-terminus containing PQS2 and 3xHA tags and a selection cassette |
84917 | pAav-MCS-PQS2-3xHA | rAAV-based template for genome engineering of protein C-termini containing PQS2 and 3xHA tags and a selection cassette |