Visualization of Compartmentalized Kinase Activity Dynamics Using Adaptable BimKARs.
Depry C, Mehta S, Li R, Zhang J
Chem Biol. 2015 Nov 19;22(11):1470-9. doi: 10.1016/j.chembiol.2015.10.004. Epub 2015 Nov 5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 84629 | pcDNA3-Cerulean-WW-NES | Encodes N-terminal (PAABD) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted |
| 84630 | pcDNA3-ERKsubDD-Venus-NES | Encodes C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted |
| 84631 | pcDNA3-ERKsub(TA)DD-Venus-NES | Encodes negative-control C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted |
| 84632 | pcDNA3-JNKAR1-NES | Unimolecular, FRET-based JNK activity reporter; cytosol targeted |
| 84633 | pcDNA3-JNKsubDD-YPet-NES | Encodes C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES |
| 84634 | pcDNA3-JNKsub(TA)DD-YPet-NES | Encodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES |
| 84635 | pcDNA3-AMPKsub-YPet-NES | Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES |
| 84636 | pcDNA3-AMPKsub(TA)-YPet-NES | Encodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES |
| 84637 | pcDNA3-ABKAR-Kras | Unimolecular, FRET-based AMPK/BRSK activity reporter; plasma membrane targeted |
| 84638 | pcDNA3-AMPKsub-YPet-Kras | Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES |
| 84639 | pcDNA3-mCherry-LKB1(WT) | mCherry-tagged, wild-type LKB1 |
| 84640 | pcDNA3-mCherry-LKB1(KD) | mCherry-tagged, LKB1(K78I); kinase-dead mutant |
| 84641 | pcDNA3-mCherry-LKB1(S431A) | mCherry-tagged, LKB1(S431A); lacks C-terminal PKA phosphorylation site |