Translation dynamics of single mRNAs in live cells and neurons.
Wu B, Eliscovich C, Yoon YJ, Singer RH
Science. 2016 Jun 17;352(6292):1430-5. doi: 10.1126/science.aaf1084. Epub 2016 May 5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 84561 | pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre | pSINAPS; single molecule translation reporter containing a FLAG tag, Suntag, oxBFP, AID, beta-actin 3'UTR, and MS2V5 stem-loops |
| 84563 | pUbC-OsTIR1-myc-IRES-scFv-sfGFP | Bicistronic vector for expression of O. sativa E3 ubiquitin ligase and single chain variable fragment of GCN4 antibody fused to superfolder GFP |