A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous System and Neurodegenerative Disease.
Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
Zebrafish. 2016 Sep 15.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 74632 | p5E--3mnx1 | Can be used to drive expression specifically in zebrafish motor neurons |
| 75023 | p5E-mpeg1.1 | Can be used to drive expression in zebrafish microglia. |
| 75024 | p5E-gfap | Can be used to drive expression in zebrafish astrocytes. |
| 75025 | p5E-elavl3 | Can be used to drive pan neuronal expression in zebrafish. |
| 75148 | pME-mTagBFP | TagBFP (blue) fluorophore for use with 5’ promoters. |
| 75149 | pME-mTagBFP-CAAX | Membrane targeted TagBFP (blue) fluorophore for use with 5’ promoters. |
| 75153 | pME-mVenus-CAAX | Membrane targeted mVenus (yellow) fluorophore for use with 5’ promoters. |
| 75154 | pME-H2B-mCerulean3-P2A-mKOFP2-CAAX | Nuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters. |
| 75155 | pME-mKOFP2-CAAX | Membrane targeted KOFP2 (orange) fluorophore for use with 5’ promoters. |
| 75156 | pME-H2B-mCerulean3 | Nuclear mCerulean2 (blue) fluorophore for use with 5’ promoters. |
| 75158 | pME-loxP-mTagBFP2-stop-pA-loxP | Floxed TagBFP2 (blue) fluorophore with stop codon for inducible lines. |
| 75174 | p3E-MCS | Multiple cloning site for insertion of gene of interest for C-terminal fusions. |
| 75175 | p3E-mTagBFP | TagBFP (blue) fluorophore with stop codon for C-terminal fusions |
| 75176 | p3E-mTagBFP-CAAX | Membrane targeted TagBFP (blue) fluorophore with stop codon for C-terminal fusions |
| 75177 | p3E-H2B-mCerulean3-P2A-mKOFP2-CAAX | Nuclear mCerulean3 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with C-terminal fusions. |
| 75179 | p3E-mCherry-NS-MCS | mCherry (red) fluorophore with multiple cloning site for use with inducible N-terminal fusions. |
| 75180 | p3E-EGFP-NS-MCS | EGFP (green) fluorophore with multiple cloning site for use with inducible N-terminal fusions. |
| 75341 | pME-EGFP-NS | Multisite gateway entry clone for adding N-terminal fusions of EGFP |
| 75342 | pME-TagBFP-NS | Multisite gateway entry clone for adding N-terminal fusions of TagBFP |
| 75343 | pME-TagRFPt-NS | Multisite gateway entry clone for adding N-terminal fusions of TagRFPt |
| 78250 | pTol2p2A--3mnx1:mTagBFP-NS-MCS | Can be used to subclone in gene of interest into blue motor neuron expression vector. |
| 78251 | pTol2p2A--3mnx1:loxP-mTagBFP2-stop-loxP-mCherry-NS-MCS | Can be used to subclone in tagged gene of interest into inducible motor neuron expression vector. |