The CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway.
Chantranupong L, Scaria SM, Saxton RA, Gygi MP, Shen K, Wyant GA, Wang T, Harper JW, Gygi SP, Sabatini DM
Cell. 2016 Mar 24;165(1):153-64. doi: 10.1016/j.cell.2016.02.035. Epub 2016 Mar 10.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 84487 | pRK5 CASTOR1-HA Cterm | overexpression of CASTOR1 with HA tag |
| 84488 | pRK5 CASTOR1-Flag Cterm | overexpression of CASTOR1 with Flag tag |
| 84489 | pRK5 CASTOR2-HA Cterm | overexpression of CASTOR2 with HA tag |
| 84490 | pRK5 CASTOR2-Flag Cterm | overexpression of CASTOR2 with Flag tag |
| 84491 | pRK5-Flag CASTOR1 ACT1 | overexpression of CASTOR1 ACT1 domain (aa 1-169) |
| 84492 | pRK5-HA CASTOR1 ACT2 | overexpression of CASTOR1 ACT2 domain (aa 169-end) |
| 84493 | pRK5-Flag CASTOR2 ACT1 | overexpression of CASTOR2 ACT1 domain (aa 1-171) |
| 84494 | pRK5-HA CASTOR2 ACT2 | overexpression of CASTOR2 ACT2 domain (aa 171-end) |
| 84495 | pRK5-Flag CASTOR1 I280A | overexpression of CASTOR1 I280A mutant |
| 84496 | pLJC6-Flag CASTOR1 | Lentiviral expression of CASTOR1 |
| 84497 | pLJC6-Flag CASTOR1 I280A | Lentiviral expression of CASTOR2 I280A mutant |