Editing DNA Methylation in the Mammalian Genome.
Liu XS, Wu H, Ji X, Stelzer Y, Wu X, Czauderna S, Shu J, Dadon D, Young RA, Jaenisch R
Cell. 2016 Sep 22;167(1):233-247.e17. doi: 10.1016/j.cell.2016.08.056.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 84475 | Fuw-dCas9-Tet1CD | Lentiviral construct to express dCas9 fused with the catalytic domain of Tet1 |
| 84476 | Fuw-dCas9-Dnmt3a | Lentiviral construct to express dCas9 fused with Dnmt3a |
| 84477 | pgRNA-modified | guide RNA expression lentiviral construct |
| 84478 | Fuw-dCas9-Dnmt3a_IM | Lentiviral construct to express dCas9 fused with an inactive Dnmt3a catalytic domain (E664A, E756A) |
| 84479 | Fuw-dCas9-Tet1CD_IM | Lentiviral construct to express dCas9 fused with an inactive catalytic domain of Tet1 (H1672Y, D1674A) |
| 84569 | Fuw-dCas9-Dnmt3a-P2A-tagBFP | Lentiviral construct to express dCas9 fused with Dnmt3a-P2A-tagBFP |
| 84570 | 138-dCas9-Dnmt3a | PiggyBac transposon system construct with dCas9-Dnmt3a |