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Investigating essential gene function in Mycobacterium tuberculosis using an efficient CRISPR interference system.

Singh AK, Carette X, Potluri LP, Sharp JD, Xu R, Prisic S, Husson RN
Nucleic Acids Res. 2016 Jul 12. pii: gkw625. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
84379pRH2502Expression of dcas9 D10A H840A from a TetR-regulated uvtetO promoter
84380pRH2521Expression of sgRNA from a imyc promoter (Pimyc)

Antibodies from Article