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Vimentin Intermediate Filaments Template Microtubule Networks to Enhance Persistence in Cell Polarity and Directed Migration.

Gan Z, Ding L, Burckhardt CJ, Lowery J, Zaritsky A, Sitterley K, Mota A, Costigliola N, Starker CG, Voytas DF, Tytell J, Goldman RD, Danuser G
Cell Syst. 2016 Sep 21. pii: S2405-4712(16)30262-9. doi: 10.1016/j.cels.2016.08.007. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
62220pCGS652Mammalian expression TALEN vector with N152, C+18 truncations
62849pRS-mEmerald-VIMDonor vector for gene editing at human VIM locus
62850pRS-mTagRFP-TUBA1BDonor vector for gene editing at human TUBA1B locus
62852pGoldyTALEN-VIM-TAL-2TALEN mediated gene editing at human VIM locus
62853pGoldyTALEN-TUBA1B-TAL-1TALEN mediated gene editing at human TUBA1B locus
62854pGoldyTALEN-TUBA1B-TAL-2TALEN mediated gene editing at human TUBA1B locus
62855pGoldyTALEN-VIM-TAL-1TALEN mediated gene editing at human VIM locus

Antibodies from Article