An Intein-based Strategy for the Production of Tag-free Huntingtin Exon 1 Proteins Enables New Insights into the Polyglutamine Dependence of Httex1 Aggregation and Fibril Formation.
Vieweg S, Ansaloni A, Wang ZM, Warner JB, Lashuel HA
J Biol Chem. 2016 Jun 3;291(23):12074-86. doi: 10.1074/jbc.M116.713982. Epub 2016 Mar 21.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 84347 | pTWIN1-His6-Ssp-Httex1-7Q | Expression of the human Huntingtin Exon1 protein containing 7Q in E.coli |
| 84348 | pTWIN1-His6-Ssp-Httex1-15Q | Expression of the human Huntingtin Exon1 protein containing 15Q in E.coli |
| 84349 | pTWIN1-His6-Ssp-Httex1-23Q | Expression of the human Huntingtin Exon1 protein containing 23Q in E.coli |
| 84350 | pTWIN1-His6-Ssp-Httex1-29Q | Expression of the human Huntingtin Exon1 protein containing 29Q in E.coli |
| 84351 | pTWIN1-His6-Ssp-Httex1-37Q | Expression of the human Huntingtin Exon1 protein containing 37Q in E.coli |
| 84352 | pTWIN1-His6-Ssp-Httex1-43Q | Expression of the human Huntingtin Exon1 protein containing 43Q in E.coli |