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Quantitative Single-Cell Analysis of Signaling Pathways Activated Immediately Downstream of Histamine Receptor Subtypes.

van Unen J, Rashidfarrokhi A, Hoogendoorn E, Postma M, Gadella TW Jr, Goedhart J
Mol Pharmacol. 2016 Sep;90(3):162-76. doi: 10.1124/mol.116.104505. Epub 2016 Jun 29. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
84325pH1R-mCherry-N1Histamine-1 receptor tagged with mCherry
84326pH2R-mCherry-N1Histamine-2 receptor tagged with mCherry
84327pH3R-mCherry-N1Histamine-3 receptor tagged with mCherry
84328pH4R-mCherry-N1Histamine-4 receptor tagged with mCherry
84329pP2A-mCherry-N1Encodes P2A-mCherry preceded by a multiple cloning sequence. Enables bicistronic expression of protein of interest and mCherry
84330pH1R-P2A-mCherry-N1Co-expresses (untagged) Histamine-1 receptor and mCherry
84331pH2R-P2A-mCherry-N1Co-expresses (untagged) Histamine-2 receptor and mCherry
84332pH3R-P2A-mCherry-N1Co-expresses (untagged) Histamine-3 receptor and mCherry
84333pH4R-P2A-mCherry-N1Co-expresses (untagged) Histamine-4 receptor and mCherry

Antibodies from Article