CRISPR-Cas system enables fast and simple genome editing of industrial Saccharomyces cerevisiae strains.
Stovicek V, Borodina I, Forster J
Metab Eng Commun. 2015 Mar 20;2:13-22. doi: 10.1016/j.meteno.2015.03.001. eCollection 2015 Dec.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 83946 | pCfB2312 (TEF1p-Cas9-CYC1t_kanMX) | Cas9-carrying vector with kanMX marker |
| 83947 | pCfB2311 (SNR52p-gRNA.ADE2-SUP4t_natMX) | gRNA cassette-carrying vector with natMX marker |