Coordinated nuclease activities counteract Ku at single-ended DNA double-strand breaks.
Chanut P, Britton S, Coates J, Jackson SP, Calsou P
Nat Commun. 2016 Sep 19;7:12889. doi: 10.1038/ncomms12889.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 82030 | pICE-HA-CtIP-siR-WT | Plasmid for constitutive or doxycycline-inducible expression of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression. |
| 82031 | pICE-HA-CtIP-siR-S664A-S679A-S745A | Plasmid for constitutive or doxy-inducible expression of S664A+S679A+S745A human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression. |
| 82032 | pICE-HA-CtIP-siR-N289A-H290A | Plasmid for constitutive or doxy-inducible expression of N289A+H290A mutant of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression. |
| 82033 | pICE-HA-MRE11-WT | Plasmid for constitutive or doxycycline-inducible expression of wild-type human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression. |
| 82034 | pICE-HA-MRE11-H129N | Plasmid for constitutive or doxycycline-inducible expression of H129N nuclease-dead mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression. |
| 82035 | pICE-HA-MRE11-H63S | Plasmid for constitutive or doxycycline-inducible expression of H63S mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression. |
| 82036 | pICE-HA-MRE11-H63D | Plasmid for constitutive or doxycycline-inducible expression of H63D exonuclease-dead mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression. |