Cpf1 nucleases demonstrate robust activity to induce DNA modification by exploiting homology directed repair pathways in mammalian cells.
Toth E, Weinhardt N, Bencsura P, Huszar K, Kulcsar PI, Talas A, Fodor E, Welker E
Biol Direct. 2016 Sep 14;11:46. doi: 10.1186/s13062-016-0147-0.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 74041 | pTE4396 | Expresses human codon-optimized AsCpf1 and As crRNA. |
| 74042 | pTE4398 | Expresses human codon-optimized LbCpf1 and Lb crRNA. |
| 80441 | WN10151 | Expresses human codon-optimized dead (832A) LbCpf1 |
| 89050 | pTE4254 | Expresses St chimeric gRNA and human codon-optimized StCas9 |
| 89051 | pKS7107 | Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9 |
| 89052 | pWN10042 | GF-ori-FP plasmid for GFxFP assay |