Targeted DNA demethylation in vivo using dCas9-peptide repeat and scFv-TET1 catalytic domain fusions.

Morita S, Noguchi H, Horii T, Nakabayashi K, Kimura M, Okamura K, Sakai A, Nakashima H, Hata K, Nakashima K, Hatada I
Nat Biotechnol. 2016 Aug 29. doi: 10.1038/nbt.3658. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
82559	pPlatTET-gRNA2	All in one vector which contains Cas9 peptide array (linker length: 22aa), antibody-sfGFP-TET1CD, and gRNA expression system.
82560	pCAG-dCas9-5xPlat2AflD	Expression vector for Cas9 peptide array (linker length: 22aa).
82561	pCAG-scFvGCN4sfGFPTET1CD	Expression vector for antibody-sfGFP-TET1CD.