Identification of Novel Nuclear Factor of Activated T Cells (NFAT)-Associated Proteins in T cells.
Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel H, Radbruch A, Krause E, Baumgrass R
J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 74046 | pMSCV-FLAG-hIKAROS-IRES-GFP | retroviral plasmid for expression of FLAG-tagged human Ikaros corresponding to cDNA from XM_011515058.1 |
| 74047 | pMSCV-FLAG-hHelios-IRES-GFP | retroviral plasmid for expression of FLAG-tagged human Helios (IKZF2) corresponding to cDNA from NM_001079526.1 |
| 74049 | pMIG-hNFATc1/bC | retroviral expression plasmid for human NFATc1/bC |
| 74050 | pMIG-hNFATc2 | retroviral expression plasmid for human NFATc2/C |
| 74051 | pMIG-hH4-AVITEV | retroviral expression plasmid for human histone H4 with C-terminal BirA-biotinylation signal and TEV celavage site |
| 74052 | pMSCV-AVITEV-GFP | retroviral expression plasmid for GFP with N-terminal BirA-biotinylation signal and TEV celavage site |
| 74053 | pMIG-hNFATc2/C(RIT)-FLAG-AVITEV | retroviral expression plasmid for human NFATc2/C (with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage site |
| 74054 | pMIG-hNFATc2/C(1-686)AVITEV | retroviral expression plasmid for human NFATc2/C (without C-terminal region) with C-terminal BirA-biotinylation signal and TEV celavage site |
| 74055 | pMIG-hNFATc2/C(1-686, RIT)FLAG-AVITEV | retroviral expression plasmid for human NFATc2/C(1-686, RIT) (without C-terminal region, with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage site |
| 74056 | pMSCV-BirA-P2A-mCherry | retroviral expression plasmid for selfcleaving E.coli biotin ligase BirA-P2A-mCherry fusion protein |
| 74057 | pMIG-AVITEV- hNFATc1/aA | retroviral expression plasmid for human NFATc1/aA with N-terminal BirA-biotinylation signal and TEV celavage site |
| 74058 | pMIG-hNFATc2/C - AVITEV | retroviral expression plasmid for human NFATc2/C with C-terminal BirA-biotinylation signal and TEV celavage site |
| 74059 | pMIG-hNFATc1/bC - AVITEV | retroviral expression plasmid for human NFATc1/bC with C-terminal BirA-biotinylation signal and TEV celavage site |
| 75232 | NFATc2-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP) | CRISPR/Cas9 plasmid against human NFATc2 |
| 75233 | NFATc2-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP) | CRISPR/Cas9 plasmid against human NFATc2 |
| 75234 | NFATc2-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP) | CRISPR/Cas9 NICKASE plasmid against human NFATc2 (1/2) |
| 75235 | NFATc2-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP) | CRISPR/Cas9 NICKASE plasmid against human NFATc2 (2/2) |
| 75236 | NFATc1-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP) | CRISPR/Cas9 plasmid against human NFATc1 |
| 75237 | NFATc1-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP) | CRISPR/Cas9 plasmid against human NFATc1 |
| 75238 | NFATc1-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP) | CRISPR/Cas9 NICKASE plasmid against human NFATc1 (1/2) |
| 75239 | NFATc1-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP) | CRISPR/Cas9 NICKASE plasmid against human NFATc1 (2/2) |
| 75240 | Ikaros-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP) | CRISPR/Cas9 plasmid against human Ikaros |
| 75241 | Ikaros-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP) | CRISPR/Cas9 plasmid against human Ikaros |
| 75242 | Ikaros-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP) | CRISPR/Cas9 NICKASE plasmid against human Ikaros (1/2) |
| 75243 | Ikaros-CRISPR-Nick2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP) | CRISPR/Cas9 NICKASE plasmid against human Ikaros (2/2) |
| 75244 | Helios-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP) | CRISPR/Cas9 plasmid against human Helios |
| 75246 | Helios-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP) | CRISPR/Cas9 NICKASE plasmid against human Helios (1/2) |
| 75247 | Helios-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP) | CRISPR/Cas9 NICKASE plasmid against human Helios (2/2) |
| 75248 | pQCXIX_sh_hBcl10_#1 | retroviral shRNA vector against human BCL10, expresses GFP for transduction control |
| 75252 | pQCXIX_sh_HOIP/RNF31_#6 | retroviral shRNA vector against human HOIP/RNF31, expresses GFP for transduction control |
| 75253 | pQCXIX_sh_hBcl10_#16 | retroviral shRNA vector against human BCL10, expresses GFP for transduction control |
| 75254 | pQCXIX_sh_hBcl10_#2 | retroviral shRNA vector against human BCL10, expresses GFP for transduction control |
| 75255 | pQCXIX_sh_hMIB2_#9 | retroviral shRNA vector against human MIB2, expresses GFP for transduction control |
| 75256 | pMSCV40-EF1a-hBCL10-SO | retroviral expression vector for expression of StrepOne-Tagged human BCL10 |
| 75257 | pMSCV40-EF1a-hBCL10(1-114)-SO | retroviral expression vector for expression of StrepOne-Tagged human BCL10 (AS 1-116) |
| 75258 | pMSCV40-EF1a-hBCL10(1-185)-SO | retroviral expression vector for expression of StrepOne-Tagged human BCL10 (AS 1-185) |