Controlling heterologous gene expression in yeast cell factories on different carbon substrates and across the diauxic shift: a comparison of yeast promoter activities.

Peng B, Williams TC, Henry M, Nielsen LK, Vickers CE
Microb Cell Fact. 2015 Jun 26;14:91. doi: 10.1186/s12934-015-0278-5. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
65451	pILGFP3	pILGFP3 was designed to test the promoter activity using the yeast enhanced green fluorescent protein (yEGFP) as reporter.
65452	pITGFP3	pITGFP3 was designed to test the promoter activity using the destabilized yeast enhanced green fluorescent protein (yEGFP-CLN2PEST) as reporter.
83538	pILGFP77	Used to evaluate the expression output of RPL8B promoter with yEGFP used as the reporter gene.
83539	pILGFP78	Used to evaluate the expression output of SSB1 promoter with yEGFP used as the reporter gene.
83540	pILGFP80	Used to evaluate the expression output of RPL3promoter with yEGFP used as the reporter gene.
83541	pILGFP82	Used to evaluate the expression output of YEF3 promoter with yEGFP used as the reporter gene.
83542	pILGFP84	Used to evaluate the expression output of SSA1 promoter with yEGFP used as the reporter gene.
83543	pILGFP86	Used to evaluate the expression output of RPL4A promoter with yEGFP used as the reporter gene.
83544	pILGFP88	Used to evaluate the expression output of RPL15A promoter with yEGFP used as the reporter gene.
83545	pILGFPE9	Used to evaluate the expression output of TEF1 promoter with yEGFP used as the reporter gene.
83547	pILGFP90	Used to evaluate the expression output of TEF2 promoter with yEGFP used as the reporter gene.
83548	pILGFP91	Used to evaluate the expression output of PGK1 promoter with yEGFP used as the reporter gene.
83549	pILGFP92	Used to evaluate the expression output of TDH3 promoter with yEGFP used as the reporter gene.
83550	pILGFP95	Used to evaluate the expression output of PDA1 promoter with yEGFP used as the reporter gene.
83551	pILGFP97	Used to evaluate the expression output of CYC1 promoter with yEGFP used as the reporter gene.
83552	pILGFPH0	Used to evaluate the expression output of TPS1 promoter with yEGFP used as the reporter gene.
83553	pILGFPH3	Used to evaluate the expression output of HXT7 promoter with yEGFP used as the reporter gene.
83554	pILGFPH7	Used to evaluate the expression output of ADH2 promoter with yEGFP used as the reporter gene.
83555	pILGFPI7	Used to evaluate the expression output of CUP1 promoter with yEGFP used as the reporter gene.
83556	pILGFPB5A	Used to evaluate the expression output of GAL1 promoter with yEGFP used as the reporter gene.
83560	pITGFP89	Used to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.