A multifunctional AAV-CRISPR-Cas9 and its host response.
Chew WL, Tabebordbar M, Cheng JK, Mali P, Wu EY, Ng AH, Zhu K, Wagers AJ, Church GM
Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 80930 | pAAV-SMVP-Cas9N | Expresses Cas9N in mammalian cells. |
| 80931 | pAAV-SMVP-Cas9C | Expresses Cas9C in mammalian cells. |
| 80932 | pAAV-CASI-Cas9C | Expresses Cas9C in mammalian cells; derived from pZac2.1 with CASI promoter. |
| 80933 | pAAV-CMV-Cas9C-VPR | Expresses Cas9C-VPR in mammalian cells. |
| 80934 | pSMVP-Cas9N | Plasmid that expresses Cas9N in mammalian cells. |
| 80935 | pSMVP-Cas9C-P2A-turboGFP | Plasmid that expresses Cas9C in mammalian cells; co-expresses turboGFP. |
| 80936 | pAAV-SMVP-Cas9N-U6-gRNA M3 | Expresses Cas9N in mammalian cells; expresses gRNA M3 for Mstn cleavage. |
| 80937 | pAAV-SMVP-Cas9N-U6-gRNA M4 | Expresses Cas9N in mammalian cells; expresses gRNA M4 for Mstn cleavage. |
| 80938 | pAAV-SMVP-Cas9N-U6-gRNA TdL | Expresses Cas9N in mammalian cells; expresses gRNA TdL for Ai9 cleavage. |
| 80939 | pSMVP-Cas9C | Plasmid that expresses Cas9C in mammalian cells. |
| 80940 | pAAV-SMVP-Cas9N-U6-gRNA TdR | Expresses Cas9N in mammalian cells; expresses gRNA TdR for Ai9 cleavage. |
| 80941 | pSMVP-Cas9FL-P2A-turboGFP | Plasmid that expresses Cas9FL in mammalian cells; co-expresses turboGFP. |
| 80942 | pAAV-CASI-Cas9C-P2A-turboGFP | Expresses Cas9C in mammalian cells; co-expresses turboGFP. |
| 80943 | pAAV-SMVP-Cas9N-U6-gRNA P1 | Expresses Cas9N in mammalian cells; expresses gRNA P1 for Pd-l1 binding. |
| 80944 | pAAV-SMVP-Cas9N-U6-gRNA P2 | Expresses Cas9N in mammalian cells; expresses gRNA P2 for Pd-l1 binding |