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Proximity-Directed Labeling Reveals a New Rapamycin-Induced Heterodimer of FKBP25 and FRB in Live Cells.

Lee SY, Lee H, Lee HK, Lee SW, Ha SC, Kwon T, Seo JK, Lee C, Rhee HW
ACS Cent Sci. 2016 Aug 24;2(8):506-16. doi: 10.1021/acscentsci.6b00137. Epub 2016 Aug 12. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
83415pCDNA5_FRB-pBirA-HAproximity-directed biotin labeling on the FRB domain of mTOR in mammalian cell
83418pCDNA5_FKBP25-V5-APEX2Expressed FKBP25/FKBP3-V5-APEX2 in mammalian cell

Antibodies from Article