A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes.
Sidik SM, Huet D, Ganesan SM, Huynh MH, Wang T, Nasamu AS, Thiru P, Saeij JP, Carruthers VB, Niles JC, Lourido S
Cell. 2016 Sep 8;166(6):1423-1435.e12. doi: 10.1016/j.cell.2016.08.019. Epub 2016 Sep 2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 80322 | pU6-SAG1-DHFR | Encodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamine |
| 80323 | pCas9-CAT | Encodes Cas9 and chloramphenicol acetyltransferase (CAT) |
| 80324 | pU6-Decoy | Encodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondii |
| 80329 | pU6-DHFR | Allows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamine |
| 80636 | Lourido Toxoplasma CRISPR Library V1 | Plasmid sgRNA library containing ten guides against each of the 8,158 predicted Toxoplasma gondii protein coding genes. |