Nico Dissmeyer Lab: artificial N-end rule substrates

Unpublished

Plasmids from Article

ID	Plasmid	Purpose
83383	His8:MBP-tev-Gly-eK-Flv	bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
83384	His8:MBP-tev-Phe-eK-Flv	bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
83385	His8:MBP-tev-Arg-eK-Flv	bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
83386	His8:MBP-tev-Leu-eK-Flv	bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
83387	His8:MBP-tev-Lys-eK-Flv	bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
83388	His8:MBP-tev-His-eK-Flv	bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
83389	His8:MBP-tev-Asp-eK-Flv	bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
83390	His8:MBP-tev-Trp-eK-Flv	bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies