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Single-cell RNA-seq reveals novel regulators of human embryonic stem cell differentiation to definitive endoderm.

Chu LF, Leng N, Zhang J, Hou Z, Mamott D, Vereide DT, Choi J, Kendziorski C, Stewart R, Thomson JA
Genome Biol. 2016 Aug 17;17(1):173. doi: 10.1186/s13059-016-1033-x. (Link opens in a new window) PubMed

Plasmids from Article

ID Plasmid Purpose
83344T-2A-EGFP-PGK-PuroDonor vector to knocin EGFP into human Brachyury(T) locus
83346T-STOP sgRNA-1The sgRNA at the stop codon of human Brachyury(T) locus

Antibodies from Article