Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit.
Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, Boch J, Stuttmann J
Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
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Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
79440 | pDGE1 | recipient plasmid [multiplexing]; p35S:Cas9, nptII |
79441 | pDGE2 | recipient plasmid [multiplexing]; p35S:Cas9, BASTA |
79442 | pDGE3 | recipient plasmid [multiplexing]; pPcUbi:Cas9, nptII |
79443 | pDGE4 | recipient plasmid [multiplexing]; pPcUbi:Cas9, BASTA |
79444 | pDGE62 | recipient plasmid [single nuclease]; p35S:Cas9, nptII |
79445 | pDGE63 | recipient plasmid [single nuclease]; p35S:Cas9, BASTA |
79446 | pDGE64 | recipient plasmid [single nuclease]; pPcUbi:Cas9, nptII |
79447 | pDGE65 | recipient plasmid [single nuclease]; pPcUbi:Cas9, BASTA |
79448 | pDGE144 | recipient plasmid [multiplexing]; p35S:Cas9, FAST, BASTA |
79449 | pDGE146 | recipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, BASTA |
79450 | pDGE148 | recipient plasmid [multiplexing]; pDD45:Cas9, FAST, BASTA |
79451 | pDGE151 | recipient plasmid [multiplexing]; pICU2:Cas9, FAST, BASTA |
79452 | pDGE160 | recipient plasmid [multiplexing]; p35S:Cas9, FAST, nptII |
79453 | pDGE161 | recipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, nptII |
79454 | pDGE164 | recipient plasmid [multiplexing]; pDD45:Cas9, FAST, nptII |
79455 | pDGE165 | recipient plasmid [multiplexing]; pICU2:Cas9, FAST, nptII |
79456 | pDGE166 | recipient plasmid [multiplexing]; p35S:Cas9, FAST, Hygro |
79457 | pDGE167 | recipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, Hygro |
79458 | pDGE168 | recipient plasmid [multiplexing]; pDD45:Cas9, FAST, Hygro |
79459 | pDGE169 | recipient plasmid [multiplexing]; pICU2:Cas9, FAST, Hygro |
79461 | pDGE5 | sgRNA shuttle vector; module 1 |
79462 | pDGE7 | sgRNA shuttle vector; module 2 |
79463 | pDGE8 | sgRNA shuttle vector; module 2E |
79465 | pDGE9 | sgRNA shuttle vector; module 3 |
79466 | pDGE10 | sgRNA shuttle vector; module 4 |
79467 | pDGE11 | sgRNA shuttle vector; module 4E |
79469 | pDGE12 | sgRNA shuttle vector; module 5 |
79471 | pDGE13 | sgRNA shuttle vector; module 6 |
79473 | pDGE14 | sgRNA shuttle vector; module 7 |
79474 | pDGE15 | sgRNA shuttle vector; module 8E |
79476 | pDGE6 | sgRNA shuttle vector; module 1E |
80578 | pDGE76 | recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, nptII |
80579 | pDGE77 | recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, BASTA |
80580 | pDGE78 | recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, nptII |
80581 | pDGE79 | recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, BASTA |
80582 | pJOG250 | recipient plasmid [multiplexing, activator]; p35S:Cas9 D10A/N863A-Hax3CT, nptII |
80583 | pJOG251 | recipient plasmid [multiplexing, activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTA |
80584 | pJOG252 | recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, nptII |
80585 | pJOG253 | recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTA |
80586 | pJOG292 | empty vector (lacZ) for 2nd generation recipient assembly |
80587 | pJOG294 | dummy module (ccdB) for 2nd generation recipient assembly |