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Tagging of endogenous genes in a Toxoplasma gondii strain lacking Ku80.

Huynh MH, Carruthers VB
Eukaryot Cell. 2009 Apr;8(4):530-9. doi: 10.1128/EC.00358-08. Epub 2009 Feb 13. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
83114pYFP.LIC.DHFRPlasmid contains LIC cassette upstream of an in-frame copy of YFP and DHFR-TS selectable marker cassette for endogenous tagging on the gene locus.
83128pYFP.LIC.HXGPRTPlasmid contains LIC cassette upstream of an in-frame copy of YFP and HXGPRT selectable marker cassette for endogenous tagging on the gene locus.
83364pmCherry.LIC.HXGPRTPlasmid contains LIC cassette upstream of an in-frame copy of mCherry and HXGPRT selectable marker cassette for endogenous tagging on the gene locus
83365ptdTomato.LIC.HXGPRTPlasmid contains LIC cassette upstream of an in-frame copy of tdTomato and HXGPRT selectable marker cassette for endogenous tagging on the gene locus
83366pCFP.HXGPRTPlasmid contains LIC cassette upstream of an in-frame copy of CFP and HXGPRT selectable marker cassette for endogenous tagging on the gene locus
83367pSF-TAP.LIC.HXGPRTPlasmid contains LIC cassette upstream of an in-frame copy of SF-TAP and HXGPRT selectable marker cassette for endogenous tagging on the gene locus

Antibodies from Article