Continuous genetic recording with self-targeting CRISPR-Cas in human cells.
Perli SD, Cui CH, Lu TK
Science. 2016 Aug 18. pii: aag0511.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 81245 | Construct 1 - U6p_20nt1_WT_sgRNA | For assaying self-targeting activity via Cas9 |
| 81246 | Construct 3 - U6p_20nt1_mod2_sgRNA (stgRNA) | For assaying self-targeting activity via Cas9 |
| 81247 | Construct 7 - CMVp-Cas9-3xNLS-HSVpA | Expresses Cas9 fused to 3xNLS driven by CMV promoter. For mammalian cell expression |
| 81248 | Construct 9 - U6p_30nt_stgRNA | Expresses 30nt stgRNA |
| 81249 | Construct 10 - U6p_40nt_stgRNA | Expresses 40nt stgRNA |
| 81250 | Construct 11 - U6p_70nt_stgRNA | Expresses 70nt stgRNA |
| 81251 | Construct 12 - hUBCp_Cas9_3xNLS_p2a_puroR | Lentiviral construct for building stable cell lines expressing Cas9 via puromycin selection |
| 81252 | Construct 13 - CMVp_U6p_27nt1_GFP(+3)_RFP(+2) | Mutation based toggling reporter (MTBR) |
| 81253 | Construct 28 -hUBCp_TetR_p2a_LacI_p2a_ZeoR | Lentiviral construct for building stable cell lines expressing TetR and LacI via Zeocin selection |
| 81254 | Construct 31 - 1xTetO_H1p_20nt2_stgRNA_3xLacO_U6p_20nt3_stgRNA | For expressing Dox and IPTG inducible stgRNAs |
| 81255 | Construct 33 - NFKBRp_Cas9_3xNLS_p2a-puroR | Lentiviral construct for building stable cell lines expressing Cas9 in the presence of TNFa via puromycin selection |
| 82024 | Construct 32 - NFKBRp_mKate2_2xNLS_p2a-puroR | Lentiviral construct for building stable cell lines expressing mKate2 in the presence of TNFa via puromycin selection |