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Profiling Subcellular Protein Phosphatase Responses to Coxsackievirus B3 Infection of Cardiomyocytes.

Shah M, Smolko CM, Kinicki S, Chapman ZD, Brautigan DL, Janes KA
Mol Cell Proteomics. 2017 Apr;16(4 suppl 1):S244-S262. doi: 10.1074/mcp.O116.063487. Epub 2017 Feb 7. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
82718pCDFDuet-MKK6-EEExpresses constitutively active human MKK6. Multiple cloning site 2 is empty.
82719pCDFDuet-MKK6-EE p38alphaExpresses constitutively active human MKK6 and murine p38 alpha.
82720pGEX-4T-1 3xFlag-MAPKAPK2Expresses human MAPKAPK2
82721pGEX-4T-1 3xFlag-CREBExpresses human CREB
82722pGEX-4T-1 3xFlag-STAT1Expresses human STAT1
82723pLX304-CAR-V5Expresses V5 tagged coxsackievirus and adenovirus receptor (CAR).
87770pLX302-MKP5-V5 puroExpresses V5 tagged human MAPK phosphatase 5 (MKP5/DUSP10)
87771pLX302-MKP7-V5 puroExpresses V5 tagged human MAPK phosphatase 7 (MKP7/DUSP16)
87772pLX302-DUSP19-V5 puroExpresses V5 tagged human DUSP19
87773pLX302-JSP1-V5 puroExpresses V5 tagged human JSP1 (DUSP22)
87774pLX302-PTP-SL-V5 puroExpresses V5 tagged human PTP-SL
87775pLX302-NIPP1-V5 puroExpresses V5 tagged human NIPP1
87776pEN_TTmiRc2 3XFLAG-MKK4-EE-NLSUsed as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro
87777pEN_TTmiRc2 3XFLAG-MKK7-EE-NLSUsed as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro
87778pEN_TTmiRc2 3XFLAG-EGFP-NLSUsed as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro
87779pSLIK 3XFLAG-MKK4-EE-NLS hygroInducible expression of constitutively active mutant MKK4 with a C-terminal NLS sequence
87780pSLIK 3XFLAG-MKK7-EE-NLS hygroInducible expression of constitutively active mutant MKK7 with a C-terminal NLS sequence
87781pSLIK 3XFLAG-EGFP-NLS hygroInducible expression of EGFP with a C-terminal NLS sequence
87790tet-pLKO shMKP1.v1 puroExpresses an inducible short hairpin targeting human MKP1 sequence
87791tet-pLKO shMKP1.v2 puroExpresses an inducible short hairpin targeting human MKP1 sequence
87792tet-pLKO shMKP5.v1 puroExpresses an inducible short hairpin targeting human MKP5 sequence
87793tet-pLKO shMKP5.v2 puroExpresses an inducible short hairpin targeting human MKP5 sequence
87794tet-pLKO shMKP7.v1 puroExpresses an inducible short hairpin targeting human MKP7 sequence
87795tet-pLKO shMKP7.v2 puroExpresses an inducible short hairpin targeting human MKP7 sequence
87796tet-pLKO shDUSP19 puroExpresses an inducible short hairpin targeting human DUSP19 sequence

Antibodies from Article