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The cytoplasmic capping complex assembles on adapter protein nck1 bound to the proline-rich C-terminus of Mammalian capping enzyme.

Mukherjee C, Bakthavachalu B, Schoenberg DR
PLoS Biol. 2014 Aug 19;12(8):e1001933. doi: 10.1371/journal.pbio.1001933. eCollection 2014 Aug. (Link opens in a new window) PubMed

Plasmids from Article

ID Plasmid Purpose
82472pCDNA3-myc- mCE(Del 25C)Expresses myc tag and mRNA capping enzyme without 25 amino acid from C-terminal
82473pcDNA3/ TO myc-cCE-bio (cCE-bio)Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal biotin tagged mRNA capping enzyme, inactive form
82475pCDNA3-biotin-myc- mCEExpresses mRNA capping enzyme containing N terminal biotin and myc tag

Antibodies from Article