Identification of a cytoplasmic complex that adds a cap onto 5'-monophosphate RNA.
Otsuka Y, Kedersha NL, Schoenberg DR
Mol Cell Biol. 2009 Apr;29(8):2155-67. doi: 10.1128/MCB.01325-08. Epub 2009 Feb 17.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 82459 | pcDNA3-myc-mCE | Expression of myc epitope and murine mRNA Capping Enzyme in mammalian cells |
| 82461 | pcDNA3-myc-mCE(K294A) | Expression of myc epitope and catalytic inactive form of murine mRNA Capping Enzyme (K294A) in mammalian cells |
| 82462 | pcDNA3-myc-mCE(211-597) | Expression of myc epitope and C-terminal domain of murine mRNA Capping Enzyme in mammalian cells |
| 82463 | pcDNA3-myc NES-mCE | Expression of myc epitope and murine mRNA Capping Enzyme with Terminal nuclear export signal in mammalian cells |
| 82464 | pcDNA3-myc NES- mCE(K294A) | Expresses myc tag and catalytic inactive form of mRNA capping enzyme with N terminal Nuclear export signal (NES) |
| 82465 | pcDNA3-myc-mCE NLS | Expresses myc tag and mRNA capping enzyme without Nuclear Localization Sequence (NLS) from C terminus |
| 82466 | pcDNA3-myc NES-mCE NLS | Expresses cytoplasmic restricted form of myc tagged mRNA capping enzyme |
| 82467 | pcDNA3- myc NES-mCE(K294A) NLS | Expresses cytoplasmic restricted form of myc tagged mRNA capping enzyme |
| 82468 | pcDNA4/TO-myc NES-mCE NLS-Flag | Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme |
| 82469 | pcDNA4/TO-myc NES-mCE(K294A) NLS-Flag | Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme, inactive form |
| 82470 | pcDNA4/TO-myc-mCE | Expression of myc epitope and murine mRNA Capping Enzyme in mammalian cells |
| 82471 | pcDNA4/TO-myc- GFP-Flag | Expresses myc-GFP-Flag |