Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems.

Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A
Science. 2016 Aug 4. pii: aaf8729. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
79621	pcDNA3.1_pCMV-dCas-PmCDA1 pH1-gRNA(HPRT)	Expresses dCas9-PmCDA1 and gRNA(HPRT) in mammalian cells
79620	pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)	Expresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cells
79619	pcDNA3.1_pCMV-nCas-PmCDA1 pH1-gRNA(HPRT)	Expresses nCas9-PmCDA1 and gRNA(HPRT) in mammalian cells
79618	pRS415_pGal-nCas9(D10A)	Expresses nCas9(D10A) in yeast cells
79617	pRS315e_pGal-nCas9(D10A)-PmCDA1	Expresses nCas9(D10A)-PmCDA1 in yeast cells
79616	pRS415_pGal-nCas9(H840A)	Expresses nCas9(H840A) in yeast cells
79615	pRS315e_pGal-dCas9-PmCDA1	Expresses dCas9-PmCDA1 in yeast cells
79614	pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHL	Expresses dCas9-SH3 and PmCDA1-SHL in yeast cells
79613	pRS315e_pGal-dCas9-SH3	Expresses dCas9-SH3 in yeast cells
79612	pRS315e_pGal-PmCDA1	Expresses PmCDA1 in yeast cells