Assembly and activation of dynein-dynactin by the cargo adaptor protein Hook3.
Schroeder CM, Vale RD
J Cell Biol. 2016 Aug 1;214(3):309-18. doi: 10.1083/jcb.201604002.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 74607 | pET28a-Hook3 aa 1-160 | Expresses Hook domain of Hook3 in bacteria for purification |
| 74608 | pET28a-Hook3 aa 1-160-GCN4 | Expresses artificially dimerized Hook domain of Hook3 in bacteria for purification |
| 74609 | pET28a-Hook3 aa 1-239 | Expresses Hook domain and first coiled coil of Hook3 in bacteria for purification |
| 74614 | pET28a-Hook3 aa 1-552 | Expresses a truncation of Hook3 in bacteria for purification |
| 74616 | pET28a-Hook3 Q147A, M151A, I154A | Expresses a triple mutant truncation of Hook3 in bacteria for purification |
| 74617 | pET28a-Hook3 I136A, I139A, M142A | Expresses a triple mutant truncation of Hook3 in bacteria for purification |
| 74618 | pET28a-Hook3 D102A, E108A | Expresses a double mutant truncation of Hook3 in bacteria for purification |
| 74619 | pET28a-Hook3 N68A, W69A, K77A | Expresses a triple mutant truncation of Hook3 in bacteria for purification |