CRISPR interference as a titratable, trans-acting regulatory tool for metabolic engineering in the cyanobacterium Synechococcus sp. strain PCC 7002.
Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger BF
Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 10.1016/j.ymben.2016.07.007.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 82371 | pGCG26 | YFP expressed from pJ23119 in the glpK locus with gentamicin resistance |
| 82372 | pCas2A | dCas9 expressed from aTc-inducible promoter in acsA, A RBS: AGGAGA |
| 82373 | pCas2B | dCas9 expressed from aTc-inducible promoter in acsA, B RBS: TCGAGA |
| 82374 | pCas2C | dCas9 expressed from aTc-inducible promoter in acsA, C RBS: TGGACA |
| 82375 | pCas2D | dCas9 expressed from aTc-inducible promoter in acsA, D RBS: AGGACG |
| 82376 | pCas2E | dCas9 expressed from aTc-inducible promoter in acsA, E RBS: AGGGCG |
| 82377 | pCas2F | dCas9 expressed from aTc-inducible promoter in acsA, F RBS: TGGGCG |
| 82378 | pCas5 | sgRNA targeting YFP expressed from pJ23119 in the NS1 locus with kanamycin resistance |
| 82379 | pCas7 | dCas9 expressed from low constituve promoter, c225, in acsA |
| 82380 | pCas8 | dCas9 without a promoter in acsA |
| 82381 | pCas13 | sgRNA targeting YFP (truncated to 12 bp) expressed from pJ23119 in the NS1 locus with kanamycin resistance |
| 82382 | pCas14 | sgRNA targeting YFP (truncated to 15 bp) expressed from pJ23119 in the NS1 locus with kanamycin resistance |
| 82383 | pCas15 | sgRNA targeting YFP (truncated to 18 bp) expressed from pJ23119 in the NS1 locus with kanamycin resistance |
| 82384 | pCas27 | sgRNA targeting YFP expressed from pJ23117 in the NS1 locus with kanamycin resistance |
| 82385 | pCas28 | sgRNA targeting YFP expressed from pJ23108 in the NS1 locus with kanamycin resistance |
| 82386 | pCas34 | sgRNA targeting YFP expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance |
| 82387 | pCas35 | sgRNA targeting cpcB expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance |
| 82388 | pCas36 | sgRNA targeting ccmK expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance |
| 82389 | pCas39 | sgRNA targeting glnA expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance |
| 82390 | pCas45 | sgRNA targeting YFP with additional bases 5' end like pCas34, but from pJ23119 in the NS1 locus with kanamycin resistance |
| 82391 | pCas49 | rbcL-sfGFP expressed from PccmK2 promoter with Gmr in glpK |
| 82392 | pCas52 | sgRNA targeting YFP +52 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance |
| 82393 | pCas55 | sgRNA targeting YFP +172 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance |
| 82394 | pCas56 | sgRNA targeting YFP +46 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance |
| 82395 | pCas57 | sgRNA targeting YFP +111 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance |
| 82396 | pCas59 | sgRNA targeting YFP +787 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance |
| 82397 | pCas61 | IPTG inducible, cLac143 (Markley 2015), LDH V39R with Gmr in glpK |
| 82398 | pCas73 | Δccm:Km, used to delete ccm operon |