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Sensitive and Quantitative Three-Color Protein Imaging in Fission Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with Experimentally-Characterized In Vivo Maturation Kinetics.

Al-Sady B, Greenstein RA, El-Samad HJ, Braun S, Madhani HD
PLoS One. 2016 Aug 1;11(8):e0159292. doi: 10.1371/journal.pone.0159292. eCollection 2016. (Link opens in a new window) PubMed

Plasmids from Article

ID Plasmid Purpose
74080BAS285 C terminal mKO2 hygBRS.pombe recoded mKO2 in universal C-terminal tagging plasmid, hygB resistance.
74081BAS286 C terminal SF-GFP hygBRS.pombe recoded superfolder GFP in universal C-terminal tagging plasmid, hygB resistanc
74082BAS287 C terminal E2C hygBRS.pombe recoded E2Crimson in universal C-terminal tagging plasmid, hygB resistance.
74083BAS288 C terminal mKO2 natRS.pombe recoded mKO2 in universal C-terminal tagging plasmid, nourseothricin resistance.
74084BAS289 C terminal E2C G418RS.pombe recoded E2Crimson in universal C-terminal tagging plasmid, G418 resistance
74085BAS290 C terminal E2C natRS.pombe recoded E2Crimson in universal C-terminal tagging plasmid, nourseothricin resistance
74086BAS291 C terminal mKO2 kanRS.pombe recoded mKO2 in universal C-terminal tagging plasmid, G418 resistance.
74087BAS292 C terminal SF-GFP kanRS.pombe recoded super-folder GFP in universal C-terminal tagging plasmid, G418 resistance

Antibodies from Article