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A multi-step model for facilitated unwinding of the yeast U4/U6 RNA duplex.

Rodgers ML, Didychuk AL, Butcher SE, Brow DA, Hoskins AA
Nucleic Acids Res. 2016 Dec 15;44(22):10912-10928. doi: 10.1093/nar/gkw686. Epub 2016 Aug 2. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
81232pRS314_U6_A34G_A35GU6 telestem mutant (A34G/A35G) for in vivo studies
81235pRS314_U6_G96C_A97CU6 telestem mutant (G96C/A97C) for in vivo studies
81236pRS314_U6_U100C_U101CU6 telestem mutant (U100C/U101C) for in vivo studies
81238pRS314-U6-A34G/A35G/G96C/A97CU6 telestem mutant (A34G/A35G/G96C/A97C) for in vivo studies
81239pRS314-U6-A34G/A35G/G96C/A97C/U100C/U101CU6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U101C) for in vivo studies
82328pRS314-U6-G96C/A97C/U100C/U101CU6 telestem mutant (G96C/A97C/U100C/U101C) for in vivo studies

Antibodies from Article