Simplified CRISPR-Cas genome editing for Saccharomyces cerevisiae.
Generoso WC, Gottardi M, Oreb M, Boles E
J Microbiol Methods. 2016 Aug;127:203-5. doi: 10.1016/j.mimet.2016.06.020. Epub 2016 Jun 17.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
81191 | pRCC-K | Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance |
81192 | pRCC-N | Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance |
81193 | pDuRCC-K | Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance |
81194 | pDuRCC-N | Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance |