Biochemical reconstitution of TET1-TDG-BER-dependent active DNA demethylation reveals a highly coordinated mechanism.
Weber AR, Krawczyk C, Robertson AB, Kusnierczyk A, Vagbo CB, Schuermann D, Klungland A, Schar P
Nat Commun. 2016 Mar 2;7:10806. doi: 10.1038/ncomms10806.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 81048 | pTG-mTDGa.0 | bacterial expression of murine TDG |
| 81049 | pTG-mTDGa.1 | bacterial expression of catalytically dead murine TDG |
| 81050 | pET28-mTDGa.0 | bacterial expression of murine TDG |
| 81051 | pET28-mTDGa.1 | bacterial expression of catalytically dead murine TDG |
| 81052 | pCDF-mTET1 | bacterial expression of murine TET1 |
| 81053 | pCDF-His-mTET1CD | bacterial expression of the catalytic domain of murine TET1 |
| 81054 | pCDF-His-mTET1CD∆cat | bacterial expression of catalytic dead catalytic domain of murine TET1 |
| 81055 | pGEX4T-hLIG3 | bacterial expression of human LIG3 |
| 81056 | pACYC-mTET1-N | bacterial expression of N-terminus of murine TET1 |