Zygote-mediated generation of genome-modified mice using Streptococcus thermophilus 1-derived CRISPR/Cas system.
Fujii W, Kakuta S, Yoshioka S, Kyuwa S, Sugiura K, Naito K
Biochem Biophys Res Commun. 2016 Jun 16. pii: S0006-291X(16)30988-3. doi: 10.1016/j.bbrc.2016.06.070.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
131542 | pCAG-T3-st1CAS9-pA | Expresses S. thermophilus 1-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys |