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Development of a CRISPR-Cas9 toolkit for comprehensive engineering of Bacillus subtilis.

Westbrook AW, Moo-Young M, Chou CP
Appl Environ Microbiol. 2016 Jun 3. pii: AEM.01159-16. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
85611pAW005-2Empty gRNA delivery vector for Bacillus subtilis
85612pAW014-2Multi-gRNA delivery vector containing ugtP-gRNA.P395T for Bacillus subtilis
85613pAW016-2Cas9-tracrRNA integration vector for Bacillus subtilis
85614pAW019-2Inducible dCas9 integration vector for Bacillus subtilis

Antibodies from Article