Detection and manipulation of live antigen-expressing cells using conditionally stable nanobodies.
Tang JC, Drokhlyansky E, Etemad B, Rudolph S, Guo B, Wang S, Ellis EG, Li JZ, Cepko CL
Elife. 2016 May 20;5. pii: e15312. doi: 10.7554/eLife.15312.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 75469 | pAAV-EF1a-Flp-DOG-NW | Flp recombinase dependent on GFP (Flp-DOG) for rAAV production and expression in mammalian cells |
| 75470 | pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry | Flp-dependent Chr2-mCherry encoded in an AAV vector for rAAV production and expression in mammalian cells |
| 80084 | pCAG-Flp-DOG | Flp recombinase dependent on GFP (Flp-DOG) for expression in mammalian cells |
| 80086 | pCAG-dGBP1-TagBFP | GFP-dependent dGBP1-TagBFP, for expression in mammalian cells |
| 80087 | pBMN-dGBP1-TagBFP | MMLV retroviral vector for expression of dGBP1-TagBFP with t-hcRed as an infection/transfection marker |