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PAFAH1B1 and the lncRNA NONHSAT073641 maintain an angiogenic phenotype in human endothelial cells.

Josipovic I, Fork C, Preussner J, Prior KK, Iloska D, Vasconez AE, Labocha S, Angioni C, Thomas D, Ferreiros N, Looso M, Pullamsetti SS, Geisslinger G, Steinhilber D, Brandes RP, Leisegang MS
Acta Physiol (Oxf). 2016 Apr 28. doi: 10.1111/apha.12700. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
92430pcDNA3.1+-PAFAH1B1mammalian expression of PAFAH1B1
92431pcDNA3.1+-PAFAH1B1_R22-mammalian expression of PAFAH1B1 R22 mutant. The protein is truncated and non functional (negative control).
92432pcDNA3.1+-NONHSAT073641mammalian expression of lncRNA NONHSAT073641
92433pSpCas9(BB)-2A-Puro-Exon4-PAFAH1B1expresses Cas9 and gRNA targeting PAFAH1B1
92434pSpCas9(BB)-2A-Intron4-PAFAH1B1expresses Cas9 and gRNA targeting PAFAH1B1

Antibodies from Article