Non-canonical features of the Golgi apparatus in bipolar epithelial neural stem cells.
Taverna E, Mora-Bermudez F, Strzyz PJ, Florio M, Icha J, Haffner C, Norden C, Wilsch-Brauninger M, Huttner WB
Sci Rep. 2016 Feb 16;6:21206. doi: 10.1038/srep21206.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 105969 | hsp70:Stathmin1-mKate2 | heat shock inducible transgenesis, stathmin stmn1 orig. from addgene #31326 |
| 105970 | hsp70: mKate2 dynactin1-811 | heat shock inducible transgenesis, first coiled coil domain plus some more amino acids, truncated before second coiled coil domain, it's first 811 amino acids of dynactin subunit p150 glued, has dominant negative effect on dynein function |
| 105971 | pME mKate2-dynactin1-811 | entry clone for gateway cloning, p150Glued subunit of dynactin, cofactor of dynein, dominant negative truncation, amino acids 1-811 |
| 105972 | hsp70: mKate2-DN Kif5a | heat shock inducible transgenesis |
| 105973 | p3E DN Kif5a | 3' element for Gateway cloning, 5 single nucleotide mutations compared to the reference sequence, but none of them change the amino acidtruncation aa416-1033(end) 1854 bp |