Non-canonical features of the Golgi apparatus in bipolar epithelial neural stem cells.

Taverna E, Mora-Bermudez F, Strzyz PJ, Florio M, Icha J, Haffner C, Norden C, Wilsch-Brauninger M, Huttner WB
Sci Rep. 2016 Feb 16;6:21206. doi: 10.1038/srep21206. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
105973	p3E DN Kif5a	3' element for Gateway cloning, 5 single nucleotide mutations compared to the reference sequence, but none of them change the amino acidtruncation aa416-1033(end) 1854 bp
105972	hsp70: mKate2-DN Kif5a	heat shock inducible transgenesis
105971	pME mKate2-dynactin1-811	entry clone for gateway cloning, p150Glued subunit of dynactin, cofactor of dynein, dominant negative truncation, amino acids 1-811
105970	hsp70: mKate2 dynactin1-811	heat shock inducible transgenesis, first coiled coil domain plus some more amino acids, truncated before second coiled coil domain, it's first 811 amino acids of dynactin subunit p150 glued, has dominant negative effect on dynein function
105969	hsp70:Stathmin1-mKate2	heat shock inducible transgenesis, stathmin stmn1 orig. from addgene #31326