Knock-in of large reporter genes in human cells via CRISPR/Cas9-induced homology-dependent and independent DNA repair.
He X, Tan C, Wang F, Wang Y, Zhou R, Cui D, You W, Zhao H, Ren J, Feng B
Nucleic Acids Res. 2016 May 19;44(9):e85. doi: 10.1093/nar/gkw064. Epub 2016 Feb 4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 83575 | single cut NH-donor | eGFP reporter donor containing single SA cutting site for NHEJ knock-in |
| 83576 | double cut NH-donor | eGFP reporter donor containing double SA cutting sites for NHEJ knock-in |
| 83806 | 2a-copGFP(+HA) | Express copGFP reporter for HDR-induced knock-in |
| 83807 | Sg-A | Express sgRNA targeting SA site |
| 83808 | sgRNA 1 GAPDH | Express Sg-1 sgRNA targeting GAPDH |
| 83809 | sgRNA 2 GAPDH | Express Sg-2 sgRNA targeting GAPDH |
| 87865 | ires-eGFP(+HAs) donor-1 | Express eGFP reporter for HDR-induced knock-in |