Selectable one-step PCR-mediated integration of a degron for rapid depletion of endogenous human proteins.
Sheridan RM, Bentley DL
Biotechniques. 2016 Feb 1;60(2):69-74. doi: 10.2144/000114378. eCollection 2016 Feb.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 86395 | pAc5 HA3-eDHFR-T2A-puro | for C-terminal tagging with eDHFR trimethoprim regulated degron with puro selection |
| 86396 | pAc5 HA3-eDHFR-T2A-neo | for C-terminal tagging with eDHFR trimethoprim regulated degron with neo selection |