CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a new web-tool and a modified Cas9 enzyme.
Michno JM, Wang X, Liu J, Curtin SJ, Kono TJ, Stupar RM
GM Crops Food. 2015;6(4):243-52. doi: 10.1080/21645698.2015.1106063.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 59184 | Cas9 MDC123 | 2x35S promoting Glycing Max codon optimized Cas9 with bar plant selection |
| 59187 | G10 Cas9 MDC123 | G10 promoting Glycing Max codon optimized Cas9 (N and C terminus NLS) with bar plant selection |
| 59188 | pBlu/gRNA | Serves as a shuttle vector for target oligo before insertion into Cas9 destination vector |