Engineered cellular gene-replacement platform for selective and inducible proteolytic profiling.
Morgan CW, Diaz JE, Zeitlin SG, Gray DC, Wells JA
Proc Natl Acad Sci U S A. 2015 Jul 7;112(27):8344-9. doi: 10.1073/pnas.1504141112. Epub 2015 Jun 23.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
100095 | Frb-N-TEV | SNIPer plasmid, N terminal TEV fused to Frb |
100096 | FKBP-C-TEV 219 | SNIPer plasmid, C terminal TEV fused to FKBP |
100098 | pETDuet_huCAD_ICADL (TevS) | dual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequences |