Efficient genetic encoding of phosphoserine and its nonhydrolyzable analog.
Rogerson DT, Sachdeva A, Wang K, Haq T, Kazlauskaite A, Hancock SM, Huguenin-Dezot N, Muqit MM, Fry AM, Bayliss R, Chin JW
Nat Chem Biol. 2015 Jul;11(7):496-503. doi: 10.1038/nchembio.1823. Epub 2015 Jun 1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 173897 | SepRS(2)/pSertRNA(B4)/EF-Sep | The plasmid contains an orthogonal aminoacyl-tRNA synthetase/tRNACUA pair, which directs the efficient incorporation of phosphoserine (pSer) into recombinant proteins in Escherichia coli. |