Optogenetic control of PIP3: PIP3 is sufficient to induce the actin-based active part of growth cones and is regulated via endocytosis.
Kakumoto T, Nakata T
PLoS One. 2013 Aug 7;8(8):e70861. doi: 10.1371/journal.pone.0070861. eCollection 2013.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 173860 | mCherry-PHR-iSH-T2A-CIBNcaax | Mammalian expression of mCherry-PHR-iSH-T2A-CIBNcaax (opto-PI3K) |
| 173861 | AktPH-mCherry-T2A-PHR-T2A-CIBNcaax | Mammalian expression of Akt PH-mCherry-T2A-PHR-T2A-CIBNcaax (PIP3 marker and opto-control) |
| 173862 | AktPH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax | Mammalian expression of Akt PH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax (PIP3 marker and opto-PI3K) |