Lentivirus-delivered stable gene silencing by RNAi in primary cells.
Stewart SA, Dykxhoorn DM, Palliser D, Mizuno H, Yu EY, An DS, Sabatini DM, Chen IS, Hahn WC, Sharp PA, Weinberg RA, Novina CD
RNA 2003 Apr;9(4):493-501.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 8449 | pUMVC | Packaging plasmid for producing MuLV retroviral particles. Use in conjunction with an envelope plasmid such as pCMV-VSV-G. |
| 8450 | Retrohair | |
| 8451 | Lentihair s1GFP | |
| 8452 | pMKO.1 puro | |
| 8453 | pLKO.1 puro | 3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses puromycin for selection. |
| 8454 | pCMV-VSV-G | Envelope protein for producing lentiviral and MuLV retroviral particles. Use in conjunction with a packaging vector such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral). |
| 8455 | pCMV-dR8.2 dvpr | 2nd generation lentiviral packaging plasmid. Can be used with 2nd and 3rd generation transfer vectors. Use in conjunction with an envelope plasmid such as pCMV-VSV-G. |