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Kate O'Connor-Giles Lab: Kate O'Connor-Giles Lab Donor Plasmids

Unpublished

Plasmids from Article

ID Plasmid Purpose
80801pScarlessHD-DsRed-w+Vector for generating dsDNA donors for HDR. Contains the visible marker 3xP3-DsRed flanked by piggyBac termini and GMR-white for identifying backbone incorporation.
80811pScarlessHD-sfGFP-DsRedVector containing a cassette for scarless insertion of sfGFP and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.
80820pScarlessHD-3xFLAG-DsRedVector containing a cassette for scarless insertion of 3xFLAG and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.
80822pScarlessHD-2xHA-DsRedVector containing a cassette for scarless insertion of 2xHA and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.
80898pHD-DsRed-attP-w+Vector for generating dsDNA donors for HDR. Contains an attP docking site, the visible marker 3xP3-DsRed and GMR-white for identifying backbone incorporation.
80926pHD-DsRed-w+Vector for generating dsDNA donors for HDR. Contains the visible marker 3xP3-DsRed and GMR-white for identifying backbone incorporation.
80927pHD-w+Vector for generating dsDNA donors for HDR. Empty backbone containing a StuI restriction site and GMR-white for identifying backbone incorporation.

Antibodies from Article